Protection against measles virus encephalitis by monoclonal antibodies binding to a cystine loop domain of the H protein mimicked by peptides which are not recognized by maternal antibodies.
Identifieur interne : 003E13 ( Main/Exploration ); précédent : 003E12; suivant : 003E14Protection against measles virus encephalitis by monoclonal antibodies binding to a cystine loop domain of the H protein mimicked by peptides which are not recognized by maternal antibodies.
Auteurs : D. Ziegler [Luxembourg (pays)] ; P. Fournier ; G A Berbers ; H. Steuer ; K H Wiesmüller ; B. Fleckenstein ; F. Schneider ; G. Jung ; C C King ; C P MullerSource :
- The Journal of general virology [ 0022-1317 ] ; 1996.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps monoclonaux (immunologie), Cartographie épitopique, Cellules Vero, Cystine, Disulfures, Données de séquences moléculaires, Encéphalite virale (), Humains, Hémagglutinines virales (immunologie), Lignée de cellules transformées, Rougeole (), Similitude de séquences d'acides aminés, Sites de fixation, Souris, Souris de lignée BALB C, Séquence d'acides aminés, Vaccin contre la rougeole (immunologie), Virus de la rougeole (immunologie).
- MESH :
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Hémagglutinines virales, Vaccin contre la rougeole, Virus de la rougeole.
- Animaux, Cartographie épitopique, Cellules Vero, Cystine, Disulfures, Données de séquences moléculaires, Encéphalite virale, Humains, Lignée de cellules transformées, Rougeole, Similitude de séquences d'acides aminés, Sites de fixation, Souris, Souris de lignée BALB C, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Antibodies, Viral (immunology), Binding Sites, Cell Line, Transformed, Chlorocebus aethiops, Cystine, Disulfides, Encephalitis, Viral (prevention & control), Epitope Mapping, Hemagglutinins, Viral (immunology), Humans, Measles (prevention & control), Measles Vaccine (immunology), Measles virus (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Sequence Homology, Amino Acid, Vero Cells.
- MESH :
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Hemagglutinins, Viral, Measles Vaccine.
- immunology : Measles virus.
- prevention & control : Encephalitis, Viral, Measles.
- Amino Acid Sequence, Animals, Binding Sites, Cell Line, Transformed, Chlorocebus aethiops, Cystine, Disulfides, Epitope Mapping, Humans, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Sequence Homology, Amino Acid, Vero Cells.
Abstract
After immunization with measles virus (MV) several monoclonal antibodies (MAbs) were obtained, which reacted with peptides corresponding to the amino acids 361-410 of the haemagglutinin protein (MV-H). Three of these MAbs (BH6, BH21 and BH216) inhibited haemagglutination, neutralized MV in vitro and protected animals from a lethal challenge of rodent-adapted neurotropic MV. These MAbs reacted with the 15-mer peptides H381 and H386 defining their overlapping region 386-395 as a sequential neutralizing and protective epitope, which can be imitated by a short peptide. H381 and H386 share two Cys residues (C(386)KGKIQALC(394)ENPEWA) and for optimal MAb binding of peptide (or MV) disulphide bonds were required in addition to a linear C-terminal extension. Other MAbs bound to peptides C- (BH147, BH195) and N-terminally (BH 168, BH 171) adjacent to the loop but did not neutralize or protect. When sera from measles patients or from women of child-bearing age were tested with the peptides corresponding to this haemagglutinating and neutralizing epitope (HNE), none of the sera recognized the 15-mer peptides of this region, while some reactivity was found to 30-mers homologous to different wild-type mutants. Its lack of recognition by maternal antibodies and its high degree of conservation would make the HNE loop an attractive candidate to include into a subunit vaccine, which could be administered during early childhood, independent of immune status.
DOI: 10.1099/0022-1317-77-10-2479
PubMed: 8887481
Affiliations:
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Steuer</name></author><author><name sortKey="Wiesmuller, K H" sort="Wiesmuller, K H" uniqKey="Wiesmuller K" first="K H" last="Wiesmüller">K H Wiesmüller</name></author><author><name sortKey="Fleckenstein, B" sort="Fleckenstein, B" uniqKey="Fleckenstein B" first="B" last="Fleckenstein">B. Fleckenstein</name></author><author><name sortKey="Schneider, F" sort="Schneider, F" uniqKey="Schneider F" first="F" last="Schneider">F. Schneider</name></author><author><name sortKey="Jung, G" sort="Jung, G" uniqKey="Jung G" first="G" last="Jung">G. 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Ziegler</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire National de Santé, Luxembourg.</nlm:affiliation><country xml:lang="fr">Luxembourg (pays)</country><wicri:regionArea>Laboratoire National de Santé</wicri:regionArea></affiliation></author><author><name sortKey="Fournier, P" sort="Fournier, P" uniqKey="Fournier P" first="P" last="Fournier">P. Fournier</name></author><author><name sortKey="Berbers, G A" sort="Berbers, G A" uniqKey="Berbers G" first="G A" last="Berbers">G A Berbers</name></author><author><name sortKey="Steuer, H" sort="Steuer, H" uniqKey="Steuer H" first="H" last="Steuer">H. Steuer</name></author><author><name sortKey="Wiesmuller, K H" sort="Wiesmuller, K H" uniqKey="Wiesmuller K" first="K H" last="Wiesmüller">K H Wiesmüller</name></author><author><name sortKey="Fleckenstein, B" sort="Fleckenstein, B" uniqKey="Fleckenstein B" first="B" last="Fleckenstein">B. Fleckenstein</name></author><author><name sortKey="Schneider, F" sort="Schneider, F" uniqKey="Schneider F" first="F" last="Schneider">F. Schneider</name></author><author><name sortKey="Jung, G" sort="Jung, G" uniqKey="Jung G" first="G" last="Jung">G. Jung</name></author><author><name sortKey="King, C C" sort="King, C C" uniqKey="King C" first="C C" last="King">C C King</name></author><author><name sortKey="Muller, C P" sort="Muller, C P" uniqKey="Muller C" first="C P" last="Muller">C P Muller</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibodies, Viral (immunology)</term><term>Binding Sites</term><term>Cell Line, Transformed</term><term>Chlorocebus aethiops</term><term>Cystine</term><term>Disulfides</term><term>Encephalitis, Viral (prevention & control)</term><term>Epitope Mapping</term><term>Hemagglutinins, Viral (immunology)</term><term>Humans</term><term>Measles (prevention & control)</term><term>Measles Vaccine (immunology)</term><term>Measles virus (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Cystine</term><term>Disulfures</term><term>Données de séquences moléculaires</term><term>Encéphalite virale ()</term><term>Humains</term><term>Hémagglutinines virales (immunologie)</term><term>Lignée de cellules transformées</term><term>Rougeole ()</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Séquence d'acides aminés</term><term>Vaccin contre la rougeole (immunologie)</term><term>Virus de la rougeole (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term><term>Hemagglutinins, Viral</term><term>Measles Vaccine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Anticorps monoclonaux</term><term>Hémagglutinines virales</term><term>Vaccin contre la rougeole</term><term>Virus de la rougeole</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Measles virus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Encephalitis, Viral</term><term>Measles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites</term><term>Cell Line, Transformed</term><term>Chlorocebus aethiops</term><term>Cystine</term><term>Disulfides</term><term>Epitope Mapping</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Sequence Homology, Amino Acid</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cartographie épitopique</term><term>Cellules Vero</term><term>Cystine</term><term>Disulfures</term><term>Données de séquences moléculaires</term><term>Encéphalite virale</term><term>Humains</term><term>Lignée de cellules transformées</term><term>Rougeole</term><term>Similitude de séquences d'acides aminés</term><term>Sites de fixation</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">After immunization with measles virus (MV) several monoclonal antibodies (MAbs) were obtained, which reacted with peptides corresponding to the amino acids 361-410 of the haemagglutinin protein (MV-H). Three of these MAbs (BH6, BH21 and BH216) inhibited haemagglutination, neutralized MV in vitro and protected animals from a lethal challenge of rodent-adapted neurotropic MV. These MAbs reacted with the 15-mer peptides H381 and H386 defining their overlapping region 386-395 as a sequential neutralizing and protective epitope, which can be imitated by a short peptide. H381 and H386 share two Cys residues (C(386)KGKIQALC(394)ENPEWA) and for optimal MAb binding of peptide (or MV) disulphide bonds were required in addition to a linear C-terminal extension. Other MAbs bound to peptides C- (BH147, BH195) and N-terminally (BH 168, BH 171) adjacent to the loop but did not neutralize or protect. When sera from measles patients or from women of child-bearing age were tested with the peptides corresponding to this haemagglutinating and neutralizing epitope (HNE), none of the sera recognized the 15-mer peptides of this region, while some reactivity was found to 30-mers homologous to different wild-type mutants. Its lack of recognition by maternal antibodies and its high degree of conservation would make the HNE loop an attractive candidate to include into a subunit vaccine, which could be administered during early childhood, independent of immune status.</div></front></TEI><affiliations><list><country><li>Luxembourg (pays)</li></country></list><tree><noCountry><name sortKey="Berbers, G A" sort="Berbers, G A" uniqKey="Berbers G" first="G A" last="Berbers">G A Berbers</name><name sortKey="Fleckenstein, B" sort="Fleckenstein, B" uniqKey="Fleckenstein B" first="B" last="Fleckenstein">B. Fleckenstein</name><name sortKey="Fournier, P" sort="Fournier, P" uniqKey="Fournier P" first="P" last="Fournier">P. Fournier</name><name sortKey="Jung, G" sort="Jung, G" uniqKey="Jung G" first="G" last="Jung">G. Jung</name><name sortKey="King, C C" sort="King, C C" uniqKey="King C" first="C C" last="King">C C King</name><name sortKey="Muller, C P" sort="Muller, C P" uniqKey="Muller C" first="C P" last="Muller">C P Muller</name><name sortKey="Schneider, F" sort="Schneider, F" uniqKey="Schneider F" first="F" last="Schneider">F. Schneider</name><name sortKey="Steuer, H" sort="Steuer, H" uniqKey="Steuer H" first="H" last="Steuer">H. Steuer</name><name sortKey="Wiesmuller, K H" sort="Wiesmuller, K H" uniqKey="Wiesmuller K" first="K H" last="Wiesmüller">K H Wiesmüller</name></noCountry><country name="Luxembourg (pays)"><noRegion><name sortKey="Ziegler, D" sort="Ziegler, D" uniqKey="Ziegler D" first="D" last="Ziegler">D. Ziegler</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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